Morpholino

From The School of Biomedical Sciences Wiki
(Difference between revisions)
Jump to: navigation, search
(Explanation of morpholinos: description and function)
 
Line 1: Line 1:
 -Small oligomer of synthetic nucleotide analogues that block gene expression
+
* Small oligomer of synthetic nucleotide analogues that block gene expression
 +
*25bp length
 +
*attaches to mRNA, pre/mature, to cause gene knockdown
 +
*Used as gene knockdown tool on zebra fish   
  
-25bp length<br>-attaches to mRNA, pre/mature, to cause gene knockdown
+
On mature mRNA:
  
-Used as gene knockdown tools on zebra fish&nbsp;&nbsp;&nbsp;
+
*attaches to AUG blocking ribosome interaction
  
On mature:<br>-attach to AUG blocking ribosome interaction
+
On pre-mRNA:
  
On pre-mRNA:<br>-sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein
+
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br>
  
  
  
<references /><br>
+
<references />&nbsp;Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br>
 
+
Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br>
+

Revision as of 12:20, 16 November 2017

On mature mRNA:

On pre-mRNA:


 Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.

Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox