Morpholino

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* Small oligomer of synthetic nucleotide analogues that block gene expression
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'''Description:'''
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* Small oligomer of synthetic nucleotide analogues that block gene expression  
 
*25bp length
 
*25bp length
*attaches to mRNA, pre/mature, to cause gene knockdown
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'''Function:'''
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*attaches to mRNA, pre/mature, to cause gene knockdown  
 
*Used as gene knockdown tool on zebra fish   
 
*Used as gene knockdown tool on zebra fish   
  
On mature mRNA:
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On mature mRNA:  
  
 
*attaches to AUG blocking ribosome interaction
 
*attaches to AUG blocking ribosome interaction
  
On pre-mRNA:
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On pre-mRNA:  
  
 
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br>
 
*sits on splice site, an intron-exon junction, making a double stranded RNA, blocking spliceosome which will create truncated/no protein<br>
  
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<br>
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----
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'''References
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<references />&nbsp;
  
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Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br>
  
<references />&nbsp;Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.<br>
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<references />

Revision as of 12:28, 16 November 2017

Description:

Function:

On mature mRNA:

On pre-mRNA:



References

 

Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.


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