# Nernst Equation

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− | == Application == | + | == Application == |

− | === Ussing Study of Frog Skin === | + | === Ussing Study of Frog Skin === |

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+ | In biochemistry, Nernst equation can be used to calculate the potential difference of ion between membranes. Hans H. Ussing, a Danish scientist, used a frog skin to measure the potential difference of sodium and potassium ions across the membranes with his famous invention, the Ussing chamber. |

## Revision as of 21:48, 14 November 2010

**Nernst Equation** is an equation used to calculate the electrical potential of a chemical reaction. In its equilibrium state, the Nernst equation should be zero. It also shows the direct relation between energy or potential of a cell and its participating ions. The equation is proposed by a German chemist, Walther H. Nernst (1864-1941).

## Contents |

## Equation

Nernst equation can be expressed as follows:

where

E_{cell }is the half-cell potential difference

E^{θ}_{cell }is the standard half-cell potential

R is the universal gas constant; R = 8.314471 J K^{-1} mol^{-1}

T is the thermodynamics temperature, in *Kelvin*; 0 K = -273.15^{o}C

z is the number of moles of electrons transferred between cells (defined by the valency of ions)

F is the Faraday's constant; F = 96,485.3415 C mol^{-1}

[red] is the concentration of ion that gained electrons (reduction)

[oxi] is the concentration of ion that lost electrons (oxidation)

## Membrane Potential

Nernst equation is also can be used to calculate the potential of an ion across the membrane. For potential difference of a membrane, we can manipulate the Nernst Equation as follows:

or

where

E_{m} is the potential difference of an ion between membranes_{}

R is the universal gas constant; R = 8.314471 J mol^{-1}

T is the thermodynamics temperature, in *Kelvin*; 0 K = -273.15^{o}C

z is the number of moles of electrons transferred between membranes (defined by the valency of ion)

F is the Faraday's constant; F = 96,485.3415 C mol^{-1}

[A^{-1}] is the concentration of ion outside the membrane

[A^{-1}] is the concentration of ion inside the membrane

## Application

### Ussing Study of Frog Skin

In biochemistry, Nernst equation can be used to calculate the potential difference of ion between membranes. Hans H. Ussing, a Danish scientist, used a frog skin to measure the potential difference of sodium and potassium ions across the membranes with his famous invention, the Ussing chamber.