Non-denaturing gel

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Also known as 'native' gel, non-denaturing gel is an electrophoretic gel used in polyacrylamide gel electrophoresis (PAGE). Detergents are used at a minimum to lyse cell membranes and no charged denaturing agents are used.[1] The molecules being separated remain folded, however separation does not occur as predictibly as those run in a denaturing gel as shape does not usually relate to  size and charge may not be as evenly distributed compared to proteins run in SDS polyacrylamide-gel electrophoresis

  1. http://www.ap-lab.com/native_gels.htm
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