Open state probability

From The School of Biomedical Sciences Wiki
(Difference between revisions)
Jump to: navigation, search
(Created page with "''' Open state probability, also written as O.S.P or P<sub>o , </sub>is a measure of the proportion of the total recording time that an ion channel spends in its open s...")
 
 
Line 1: Line 1:
'''&nbsp;Open state probability, also written as O.S.P or P<sub>o ,&nbsp;</sub>is a measure of the proportion of the total recording time that an ion channel spends in its open state.&nbsp;<sup></sup>'''
+
Open state probability, also written as O.S.P or P<sub>o ,&nbsp;</sub>is a measure of the proportion of the total recording time that an [[Ion_channels|ion channel]] spends in its open state.&nbsp;<sup></sup>
 +
<center>'''O.S.P = Open state time / Total recording time'''</center>
 +
''(which can be written as a percentage when the answer is multiplied by 100)''<br>
  
 +
*This is a useful way for channels to change their activity as their conductance cannot be changed but the amount of time they spend closed or opened can.
 +
*This can usually be found out by using the [[Patch clamp techniques|patch clamp technique]] and measuring the single channel recording over a certain period of time. This will be able to tell us the open state probability of that channel as well as its conductance and selectivity.<br>
  
 +
The P<sub>o</sub> value is useful when counting the number of [[Ion_channels|ion channels]] (''N'') in a cell because we are able to substitute its value (along with the rest of the values) into the following equation:
 +
<center>'''I<sub>total &nbsp;</sub>= &nbsp;N &nbsp;x &nbsp;P<sub>o</sub> &nbsp;x &nbsp;i'''<sub></sub></center>
 +
I<sub>total </sub>= &nbsp;Whole cell [[Current|current]]
  
==== O.S.P = Open state time / Total recording time ====
+
N = Number of active [[Ion channels|channels]]
  
''(which can be written as a percentage when the answer is multiplied by 100)''
+
P<sub>o</sub>&nbsp;= Open state [[Probability|probability]]
  
 
+
i = Single channel [[Current|current]]  
*This is a useful way for channels to change their activity as their conductance cannot be changed but the amount of time they spend closed or opened can.
+
*This can usually be found out by using the [[patch clamp techniques|patch clamp technique]] and measuring the single channel recording over a certain period of time. This will be able to tell us the open state probability of that channel as well as its conductance and selectivity.
+
 
+
 
+
 
+
The P<sub>o</sub> value is useful when counting the number of ion channels (''N'') in a cell because we are able to substitute its value (along with the rest of the values) into the following equation:
+
 
+
==== '''I<sub>total &nbsp;</sub>= &nbsp;N &nbsp;x &nbsp;P<sub>o</sub> &nbsp;x &nbsp;i'''<sub></sub> ====
+
 
+
 
+
I<sub>total </sub>= &nbsp;Whole cell [[current|current]]
+
 
+
N = Number of active [[Ion_channels|channels]]
+
 
+
P<sub>o</sub>&nbsp;= Open state [[probability|probability]]
+
 
+
i = Single channel [[current|current]]
+
  
 
<br>
 
<br>

Latest revision as of 07:17, 14 November 2011

Open state probability, also written as O.S.P or Po , is a measure of the proportion of the total recording time that an ion channel spends in its open state. 

O.S.P = Open state time / Total recording time

(which can be written as a percentage when the answer is multiplied by 100)

The Po value is useful when counting the number of ion channels (N) in a cell because we are able to substitute its value (along with the rest of the values) into the following equation:

Itotal  =  N  x  Po  x  i

Itotal =  Whole cell current

N = Number of active channels

Po = Open state probability

i = Single channel current


Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox