Phosphofructokinase (PFK) is a fundamental enzyme in the glycolitic pathway. As the name suggests, the enzyme acts on the intermediate compound 'phosphofructose'; its proper name being fructose-6-phosphate. The suffix 'kinase' indicates its function as a phosphorylator. The product of this enzyme-subtrate interaction is fructose 1,6 bisphosphate, which subsequently is hydrolysed to glyceralaldehyde 3 phosphate and dihydroxyacetone phosphate.
Phosphofructokinase has antagonistic action with the enzyme Fructose 1,6-bisphosphatase; a dephosphorylator.