Plasmid

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Plasmids are very useful as vectors and in [[Recombinant DNA Technology|recombinant DNA techniques]]. Desired [[Genes|genes]] can be inserted in and amplified up.  
 
Plasmids are very useful as vectors and in [[Recombinant DNA Technology|recombinant DNA techniques]]. Desired [[Genes|genes]] can be inserted in and amplified up.  
  
Examples of plasmids include the puC18, or the F plasmid. Note that the F plasmids are unusually large. This property allows large scale genetic exchange between bacteria. 
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Examples of plasmids include the [[puC18|puC18]], or the F plasmid. Note that the [[F_plasmid|F plasmids]] are unusually large. This property allows large scale genetic exchange between bacteria.   
  
Plasmid Conformation<br> [[Plasmids|Plasmids]] exist in three forms: Open circle, supercoiled and linear. Supercoiled is when the plasmid has is tightly wound round itself in order to fit inside the cell. Open circle is when there is a nick in the outer ring of the plasmid and linear is when the plasmid is cut with a [[Restriction_enzyme|restriction enzyme]].<br>When run on an agarose gel, linear runs the fastest down the gel, then supercoiled then open cirlce. <br><br>  
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=== Plasmid Conformation ===
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[[Plasmids|Plasmids]] exist in three forms: Open circle, supercoiled and linear. Supercoiled is when the plasmid has is tightly wound round itself in order to fit inside the cell. Open circle is when there is a nick in the outer ring of the plasmid and linear is when the plasmid is cut with a [[Restriction enzyme|restriction enzyme]].
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When run on an agarose gel, linear runs the fastest down the gel, then supercoiled then open cirlce. <br>  
  
 
=== References  ===
 
=== References  ===
  
<references /><references /><br>
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<references /><br>

Revision as of 03:43, 24 October 2014

Plasmids are supercoiled DNA molecules present in most species of bacteria. These are not integrated into the host chromosome and are much smaller in length.

Plasmids are not necessary for the survival of a bacteria but can contain genes that are advantageous in changing environmental conditions, an example would be antibiotic resistance genes [1].

Plasmids have no replication machinery of their own and are reliant upon the host for duplication.

Plasmids are very useful as vectors and in recombinant DNA techniques. Desired genes can be inserted in and amplified up.

Examples of plasmids include the puC18, or the F plasmid. Note that the F plasmids are unusually large. This property allows large scale genetic exchange between bacteria. 

Plasmid Conformation

Plasmids exist in three forms: Open circle, supercoiled and linear. Supercoiled is when the plasmid has is tightly wound round itself in order to fit inside the cell. Open circle is when there is a nick in the outer ring of the plasmid and linear is when the plasmid is cut with a restriction enzyme.

When run on an agarose gel, linear runs the fastest down the gel, then supercoiled then open cirlce.

References

  1. Maloy (1987), Microbial Genetics, 2nd edition, Jones and Bartlett Publishers.

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