PuC18

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PuC18 is a common [[Plasmid|bacterial plasmid]] for [[E. coli|E. Coli]]. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the [[Gene|gene]] and [[Promoter|promoter]] for [[Β-galactosidase|beta-galactosidase]], lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different [[Restriction site|restriction]] sites for [[Restriction Endonuclease|restriction endonucleases]] involved in procedures such as [[Recombination|DNA recombination]]<ref>reference</ref>.&nbsp;  
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PuC18 is a common [[Plasmid|bacterial plasmid]] for [[E. coli|E. Coli]]. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the [[Gene|gene]] and [[Promoter|promoter]] for [[Β-galactosidase|beta-galactosidase]], lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different [[Restriction site|restriction]] sites for [[Restriction Endonuclease|restriction endonucleases]] involved in procedures such as [[Recombination|DNA recombination]]<ref>M. Carr S. Plasmid pUC18 [Internet]. Mun.ca. 2014 [cited 2 December 2017]. Available from: https://www.mun.ca/biology/scarr/Plasmid_pUC18.html</ref>.&nbsp;  
  
 
Furthermore, the gene for [[Ampicillin|ampicillin]] [[Antibiotic resistance|antibiotic resistance]], amp<sup>R</sup>, is also a major feature fo puC18 alongside the main [[Origin of replication|origin of replication]], ori<sup>C</sup>.  
 
Furthermore, the gene for [[Ampicillin|ampicillin]] [[Antibiotic resistance|antibiotic resistance]], amp<sup>R</sup>, is also a major feature fo puC18 alongside the main [[Origin of replication|origin of replication]], ori<sup>C</sup>.  
  
Overall, the [[Vector|expression vector]] is 2686 base pairs in total and has a [[Molecular weight|molecular weight]] of 1.75x10<sup>6</sup> Da<ref>reference</ref>.  
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Overall, the [[Vector|expression vector]] is 2686 base pairs in total and has a [[Molecular weight|molecular weight]] of 1.75x10<sup>6</sup> Da<ref>pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/</ref>.&nbsp;
  
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'''References'''<br>
  
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Revision as of 21:01, 3 December 2017

PuC18 is a common bacterial plasmid for E. Coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination[1]

Furthermore, the gene for ampicillin antibiotic resistance, ampR, is also a major feature fo puC18 alongside the main origin of replication, oriC.

Overall, the expression vector is 2686 base pairs in total and has a molecular weight of 1.75x106 Da[2]

References

  1. M. Carr S. Plasmid pUC18 [Internet]. Mun.ca. 2014 [cited 2 December 2017]. Available from: https://www.mun.ca/biology/scarr/Plasmid_pUC18.html
  2. pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/


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