PuC18 is a common bacterial plasmid forE. coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination.
- ↑ M. Carr S. Plasmid pUC18 [Internet]. Mun.ca. 2014 [cited 2 December 2017]. Available from: https://www.mun.ca/biology/scarr/Plasmid_pUC18.html
- ↑ pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/