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PuC18 is a common bacterial plasmid for E. Coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination[1]

Furthermore, the gene for ampicillin antibiotic resistance, ampR, is also a major feature fo puC18 alongside the main origin of replication, oriC.

Overall, the expression vector is 2686 base pairs in total and has a molecular weight of 1.75x106 Da[2].

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