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 PuC18 is a common bacterial plasmid for E. Coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination

Furthermore, the gene for ampicillin antibiotic resistance, ampR, is also a major feature fo puC18 alongside the main origin of replication, oriC.

Overall, theexpression vector is 2686 base pairs in total and has a molecular weight of 1.75x106 Da.

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