PuC18

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PuC18 is a common bacterial plasmid for E. Coli. Genetic engineering allows multiple sites to be inserted into the dsDNA loop such as the gene and promoter for beta-galactosidase, lacZ. The lacZ gene contains a polylinker region that is 54 base pairs in length and contains 13 different restriction sites for restriction endonucleases involved in procedures such as DNA recombination[1]

Furthermore, the gene for ampicillin antibiotic resistance, ampR, is also a major feature fo puC18 alongside the main origin of replication, oriC.

Overall, the expression vector is 2686 base pairs in total and has a molecular weight of 1.75x106 Da[2]

References

  1. M. Carr S. Plasmid pUC18 [Internet]. Mun.ca. 2014 [cited 2 December 2017]. Available from: https://www.mun.ca/biology/scarr/Plasmid_pUC18.html
  2. pUC18 DNA [Internet]. Molecular Biology - services and products. 2017 [cited 2 December 2017]. Available from: https://www.geneon.net/products/dna-ladders/puc18-dna/


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