Pyrosequencing

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Pyrosequencing (also known as 454-sequencing) is a method of [[DNA Sequencing|DNA Sequencing]]. It involves using an [[Enzyme|enzyme]] called [[Luciferase|luciferase]], amongst others, to determine the [[Nucleotide|nucleotide]] sequence based upon the light&nbsp;emitted when a correct [[DNTP|dNTP]] binds to a template. <references />
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Pyrosequencing (also known as 454-sequencing) is a method of [[DNA Sequencing|DNA Sequencing]]. It involves using an [[Enzyme|enzyme]] called [[Luciferase|luciferase]], amongst others, to determine the [[Nucleotide|nucleotide]] sequence based upon the light&nbsp;emitted when a correct [[DNTP|dNTP]] binds to a template. <references /><ref name="null">Ronaghi M. Pyrosequencing Sheds Light On DNA Sequencing, Genome Res. 2001 11: 3-11</ref>
  
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<ref>Ronaghi M. Pyrosequencing Sheds Light On DNA Sequencing, Genome Res. 2001 11: 3-11</ref>
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Revision as of 12:51, 19 November 2010

Pyrosequencing (also known as 454-sequencing) is a method of DNA Sequencing. It involves using an enzyme called luciferase, amongst others, to determine the nucleotide sequence based upon the light emitted when a correct dNTP binds to a template. [1]


 


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