RNA polymerase II
RNA polymerase II is found in eukaryotic cells and is structurally similar to RNA polymerase I and III with all three sharing common subunits. It is used to transcribe all protein-encoding genes, and in addition, many of the small nucleolar RNAs (snoRNA) and the majority of the micro RNAs (miRNA). RNA polymerase II cannot transcribe DNA on its own: the many elements that make up the pre-initiation complex (PIC) must assemble at the site of the promoter in order for RNA polymerase II to be recruited and begin transcription. The PIC is made up of Transcription Factors: TFIID, TFIIA, TFIIB, TFIIF, TFIIE, TFIIH. RNA Pol II is also exploited for use in CRISPR technology for genome editing.
It has a very similar structure to its prokaryotic counterpart but is made up of many more subunits with a total of twelve.
- ↑ Wilhelm E et al (2012)'Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay', Methods Mol Biol. 809:63-74
- ↑ Seizl M et al. (2011). A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS One. 6 (11), e27595
- ↑ D. B. Nikolov et al. (1997). RNA polymerase II transcription initiation: A structural view. PNAS. 94 (1), 15-22.
- ↑ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023835/