Real-time Polymerase Chain Reaction
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Other names: [[Quantitative PCR|Quantitative PCR]], [[QPCR|qPCR]] | Other names: [[Quantitative PCR|Quantitative PCR]], [[QPCR|qPCR]] | ||
| − | The Real-time Polymerase Chain Reaction is an improvised version of the original [[Polymerase Chain Reaction|Polymerase Chain Reaction]] (PCR<u>)</u> developed by Kary Mullis, who received the | + | The Real-time Polymerase Chain Reaction is an improvised version of the original [[Polymerase Chain Reaction|Polymerase Chain Reaction]] (PCR<u>)</u> developed by Kary Mullis, who received the Nobel Prize in Chemistry in 1993 , and her coworkers during the mid-1980s.<ref>Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350-4.</ref> |
<br> <u>'''Monitoring of the Real-Time PCR'''</u> | <br> <u>'''Monitoring of the Real-Time PCR'''</u> | ||
The product that is formed binds to a flourescent reporter that is required for the Real-Time Polymerase Chain Reaction. It's presence is reported by flourescent. <ref>Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125</ref><br> <br> <br> <br> <references /> | The product that is formed binds to a flourescent reporter that is required for the Real-Time Polymerase Chain Reaction. It's presence is reported by flourescent. <ref>Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125</ref><br> <br> <br> <br> <references /> | ||
Revision as of 04:52, 26 November 2018
Other names: Quantitative PCR, qPCR
The Real-time Polymerase Chain Reaction is an improvised version of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis, who received the Nobel Prize in Chemistry in 1993 , and her coworkers during the mid-1980s.[1]
Monitoring of the Real-Time PCR
- ↑ Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350-4.
- ↑ Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125
