Real-time Polymerase Chain Reaction

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<br> <u>'''Monitoring of the Real-Time PCR'''</u>  
 
<br> <u>'''Monitoring of the Real-Time PCR'''</u>  
  
The product that is formed binds to a flourescent reporter that is required for the Real-Time Polymerase Chain Reaction. It's presence is reported by flourescent. <ref>Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125</ref><br> <br> <br> <br> <references />
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The product that is formed from the Real-time PCR binds to a required flourescent reporter <ref>Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125</ref>, a probe that is only developed recently to report the amplification (presence and amount)&nbsp;of PCR product in real-time.<ref>Mikael Kubista, Anders Stalberg, Tzachi Bar. Light-up-probe-based real-time Q-PCR. Geonomics and Proteomics Technologies. 2001; Proceedings volume  4264.</ref>&nbsp;The signal is very small and is almost the same as the background during the initial cycles. The amount of product would then increase and the signal would exponentially increase. At the end, the signal would saturate as the reaction does not have enough of the required components (either the primers, the flourescent reporter, or the dNTP<ref>Mikael Kubista, Anders Stalberg, Tzachi Bar. Light-up-probe-based real-time Q-PCR. Geonomics and Proteomics Technologies. 2001; Proceedings volume  4264.</ref> ) to continue with the reaction.<ref>Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125</ref><br> <br> <br> <br> <references />

Revision as of 07:06, 26 November 2018

Other names: Quantitative PCR, qPCR

The Real-time Polymerase Chain Reaction is an improvised version of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis, who received the Nobel Prize in Chemistry in 1993 , and her coworkers during the mid-1980s.[1]


Monitoring of the Real-Time PCR

The product that is formed from the Real-time PCR binds to a required flourescent reporter [2], a probe that is only developed recently to report the amplification (presence and amount) of PCR product in real-time.[3] The signal is very small and is almost the same as the background during the initial cycles. The amount of product would then increase and the signal would exponentially increase. At the end, the signal would saturate as the reaction does not have enough of the required components (either the primers, the flourescent reporter, or the dNTP[4] ) to continue with the reaction.[5]



  1. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350-4.
  2. Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125
  3. Mikael Kubista, Anders Stalberg, Tzachi Bar. Light-up-probe-based real-time Q-PCR. Geonomics and Proteomics Technologies. 2001; Proceedings volume 4264.
  4. Mikael Kubista, Anders Stalberg, Tzachi Bar. Light-up-probe-based real-time Q-PCR. Geonomics and Proteomics Technologies. 2001; Proceedings volume 4264.
  5. Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125
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