Real-time Polymerase Chain Reaction

Other names: Quantitative PCR, qPCR

The Real-time Polymerase Chain Reaction is an improvised version of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis, who received the Nobel Prize in Chemistry in 1993 , and her coworkers during the mid-1980s.^[1]

Monitoring of the Real-Time PCR

The product that is formed binds to a flourescent reporter that is required for the Real-Time Polymerase Chain Reaction. It's presence is reported by flourescent. ^[2]

1. ↑ Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350-4.
2. ↑ Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125