Real-time Polymerase Chain Reaction

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Other names: Quantitative PCR, qPCR

The Real-time Polymerase Chain Reaction is an improvised version of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis, who received the Nobel Prize in 1993 in Chemistry, and her coworkers during the mid-1980s. (1)

Monitoring of the Real-Time PCR

The product that is formed binds to a flourescent reporter that is required for the Real-Time Polymerase Chain Reaction. It's presence is reported by flourescent. (2)

  1. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350-4.
  2. Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric,The real-time polymerase chain reaction, Molecular Aspects of Medicine,Volume 27, Issues 2–3,2006,Pages 95-125,
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