SDS polyacrylamide-gel electrophoresis

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[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.Once an SDS-Page Gel electrophoresis has taken place, the fragments of [[DNA|DNA ]]then need to be visualised. This can be done through a few different methods. One way is through UV imaging, another is through adding a dye to the [[DNA|DNA]] fragments.
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[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.&nbsp;
  
 
=== References  ===
 
=== References  ===
  
 
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Revision as of 21:31, 22 October 2012

SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins by their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied [1]. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size. 

References

  1. Alberts,Bruce et al., 2008 Pg 517
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