SDS polyacrylamide-gel electrophoresis

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[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.  
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[[SDS|SDS]]-[[Polyacrylamide|polyacrylamide]]-gel [[Electrophoresis|electrophoresis]] is a technique used to seperate [[Proteins|proteins]]&nbsp;by&nbsp;their net negative charge, [[Molecular weight|molecular weight]] and shape. This is achieved through creating a gel containing cross-links of&nbsp;[[Acrylamide|acrylamide]] subunits ([[Polyacrylamide|polyacrylamide]]), which produces a matrix in which [[Proteins|proteins]] can migrate through when a electrical current is applied <ref>Alberts,Bruce et al., 2008 Pg 517</ref>. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.Once an SDS-Page Gel electrophoresis has taken place, the fragments of [[DNA|DNA ]]then need to be visualised. This can be done through a few different methods. One way is through UV imaging, another is through adding a dye to the [[DNA|DNA]] fragments.  
  
 
=== References  ===
 
=== References  ===
  
 
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Revision as of 21:27, 22 October 2012

SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins by their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied [1]. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.Once an SDS-Page Gel electrophoresis has taken place, the fragments of DNA then need to be visualised. This can be done through a few different methods. One way is through UV imaging, another is through adding a dye to the DNA fragments.

References

  1. Alberts,Bruce et al., 2008 Pg 517
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