SDS polyacrylamide-gel electrophoresis

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SDS-polyacrylamide-gel electrophoresis is a technique used to seperate proteins by their net negative charge, molecular weight and shape. This is achieved through creating a gel containing cross-links of acrylamide subunits (polyacrylamide), which produces a matrix in which proteins can migrate through when a electrical current is applied [1]. Proteins which are smaller in size migrate through the gel faster and therefore travel further on the gel than those which are larger in size.

References

  1. Alberts,Bruce et al., 2008 Pg 517
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