TFIIH

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TFIIH is a [[General transcription factor|general transcription factor]] (GTF) involved in PIC ([[Pre-Initiation Complex|Pre-Initiation Complex]]) assembly, it is added in the last stage of assembly close to the start site of transcription. The [[Helicase|helicase]] activity of TFIIH separates the template [[DNA|DNA]] strand at the start site of [[Transcription|transcription]], forming an open complex, this requires ATP hydrolysis. TFIIH has 9 subunits and main functions include:
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TFIIH is a [[General transcription factor|general transcription factor]] (GTF) involved in PIC ([[Pre-Initiation Complex|Pre-Initiation Complex]]) assembly, it is added in the last stage of assembly close to the start site of transcription. The [[Helicase|helicase]] activity of TFIIH separates the template [[DNA|DNA]] strand at the start site of [[Transcription|transcription]], forming an open complex, this requires ATP hydrolysis. TFIIH has 9 subunits and main functions include:  
  
1. Promotor melting and clearance
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1. Promotor melting and clearance  
  
2. CTD kinase activity
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2. CTD kinase activity  
  
3. DNA repair coupling
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3. DNA repair coupling  
  
As RNA  polymerase II begins transcribing, TFIIH is released from the complex along with TFIIB and TFIIE. 
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As RNA  polymerase II begins transcribing, TFIIH is released from the complex along with TFIIB and TFIIE.   
  
TFIIH can be divided into two parts: core and CAK. The core section of the GTF contains several DNA helicases including XPD and XPB. XPB plays a major role in promotor melting. The CAK module can dissociated away from the TFIIH molecule where it has other functions in the cell cycle (cdk activating kinases). 
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TFIIH can be divided into two parts: core and CAK. The core section of the GTF contains several DNA helicases including XPD and XPB. XPB plays a major role in promotor melting. The CAK module can dissociated away from the TFIIH molecule where it has other functions in the cell cycle (cdk activating kinases). The CAK module also contains one of the kinases that is involved in hosphorylation of the CTD (C-terminal domain) of RNA polymerase II. This phosphorylation is needed for promotor clearance. 
  
Mutations in the TFIIH molecule can result in three distinct genetic diseases; 
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Mutations in the TFIIH molecule can result in three distinct genetic diseases;   
  
1. Xeroderma pigmentosum<br>2. Trichothiodystrophy<br>3. Cockayne Syndrome<br>
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1. Xeroderma pigmentosum<br>2. Trichothiodystrophy<br>3. Cockayne Syndrome<br>  
  
 
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Revision as of 17:31, 28 November 2011

TFIIH is a general transcription factor (GTF) involved in PIC (Pre-Initiation Complex) assembly, it is added in the last stage of assembly close to the start site of transcription. The helicase activity of TFIIH separates the template DNA strand at the start site of transcription, forming an open complex, this requires ATP hydrolysis. TFIIH has 9 subunits and main functions include:

1. Promotor melting and clearance

2. CTD kinase activity

3. DNA repair coupling

As RNA  polymerase II begins transcribing, TFIIH is released from the complex along with TFIIB and TFIIE. 

TFIIH can be divided into two parts: core and CAK. The core section of the GTF contains several DNA helicases including XPD and XPB. XPB plays a major role in promotor melting. The CAK module can dissociated away from the TFIIH molecule where it has other functions in the cell cycle (cdk activating kinases). The CAK module also contains one of the kinases that is involved in hosphorylation of the CTD (C-terminal domain) of RNA polymerase II. This phosphorylation is needed for promotor clearance. 

Mutations in the TFIIH molecule can result in three distinct genetic diseases; 

1. Xeroderma pigmentosum
2. Trichothiodystrophy
3. Cockayne Syndrome


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