DNA BLAST - links to more data
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From the DNA BLAST results, it is possible to 'hop' to other information in the system such as who cloned the DNA, and whether or not it is involved in any diseases or conditions.
Finding the paper
We will now find the paper associated with the top hit in your BLAST search.
  1. Scroll back up to the top of the table.
Your top hit shoule be:
Top hit in the DNA BLAST search
  1. Click on the accession number, i.e. AF099138.1. If your top hit is not AF099138.1, then either scroll down to find it, or click on this link
Nucleotide database entry for the blast search
By looking just at the DNA database entry, you should be able to answer the following questions:
1) What is the accession number of the top scoring DNA in the NIH (NCBI) BLAST search?
One answer only.
   AF099134
   AF099135
   AF099136
   AF099137
   AF099138
2) When was the DNA AF099138 first cloned?
One answer only.
   1902
   1976
   1998
   1999
   2000
3) What was the species and tissue type used for cloning?
One answer only.
   human limb
   human brain
   human foreskin
   mouse limb
   mouse brain
   mouse foreskin
   mouse adipocyte
   rat limb
   rat brain
   rat foreskin
   rat adipocyte
   none of the above
From the DNA database entry, it is easy to go to the scientific paper
  1. In the DNA database entry click on the link next to the word PUBMED (it should be the number 10350628).
You should now have the abstract open in PubMed
The PubMed entry associated with the DNA database entry
From the PubMed entry you should now be able to 'hop' to the other databases to answer the following questions:
Hint: Use the 'Related Information' menu
1) What is the first page of the article in Biochim Biophys Acta.?
One answer only.
   1999
   18
   1431
   2
   525
   30
2) Does the OMIM database report the DNA as being involved in a disease?
One answer only.
   Yes
   No
   Don't know
   Don't care
   What is OMIM?
3) How many C2 domains does the protein contain?
One answer only.
   1
   2
   3
   4
   5
   6
4) Does the protein contain a potential transmembrane domain?
One answer only.
   Yes
   No
5) What is the weight (kDa) of the protein produced by this transcript?
One answer only.
   57
   88
   92
   97
   115
   121
Summary
So far you have:
  1. carried out a DNA BLAST search
  2. identified the year the DNA was sequenced
  3. identified the source of the material used for cloning
  4. identified the paper that reported the sequencing
  5. established that the DNA is not involved in a disease
We will now look at restricting DNA searches to just humans.
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