Protein Analysis - SMART
In this section, we will run a
SMART![](gifs/yellow.gif)
search to find functional domains in a protein.
You can now close all the other windows (don't close this one).
- Go to the SMART
site: http://smart.embl-heidelberg.de/![](gifs/red.gif)
The first time you go to the site you may see this:
![](gifs/protein_analysis_smart_1_start.jpg)
The screen the first time you use SMART
- If the screen looks like above, then click on 'SMART
MODE: NORMAL', i.e. the left-hand, panel.
MDAKHKNSTILGPIKAQIKAMADKVSKNALGLYHDIQVQHREYIVPISFLALILLTLANFLAGVYTFVWMLVAFKLSDVLDDVLFLRRLEKITSNVHALASNAYKQVLEESIAVESELYARFPHGEDSKQIAQIANKKKIDRWYLLDVSFRVKDKWHKKQGDMMDAKHKLGPIKAQIKAMADKVSKNALGLYHDIQVQHREYTFVWMLVAFKLSDVLDDVLFLRRLEKITSNVHALASNAYKQVLEESIAVESELYARFPHGEDSKQIAQIANKKKIDRWYLLDVSFRVKDKWHKKQGDM
(This is the same sequence as you used for the
Prosite![](gifs/yellow.gif)
search)
- Copy the above sequence
- Paste the sequence into the search box at the SMART
site
![](gifs/protein_analysis_smart_1_2016.png)
The completed SMART entry
- Click on the 'Sequence SMART
' button