Protein Analysis - Interpro - Results
Home | Index | Assessment | Blog | Wiki
The Interpro search should produce the following results.
The Interpro results - analysis run on Thursday October 12, 2017 at 8:46:15 pm
At first glance, these results don't seem to tell you much. However, if you look closely at them you will see, by mousing over the 'Detailed signature matches':
  1. a cytoplasmic domain
  2. a transmembrane spanning domain
  3. a non-cytoplasmic domain
And this suggests that the protein is membrane bound, with the N-terminal in the cell, and the C-terminal exterior.

How do I know that the N-terminal is in the cell and the C-terminal is outsie the cell?

Traditionally, we always write proteins N-terminal to C-terminal, so the N-terminal is on the left in the above image.

And DNA (and mRNA) is writing 5' to 3', so the 5' end is on the left.

When this information is combined with the data from the Prosite search, you can start to build up a picture of the protein in the cell, and this will help you plan future experiments.
1) Which region of the protein is inside the cell?
One answer only.
   The C-terminal
   The N-terminal
   All of the protein
   None of the protein
   Don't know
   Don't care
2) The C-terminal is
One answer only.
   Inside the cell
   Outside the cell
   Don't know
   Don't care
3) How many potential transmebrane spanning domain(s) does the protein contain?
One answer only.
   0
   1
   2
   3
   4
4) If the protein contains a transmebrane spanning domain, what amino acids are involved?
One answer only.
   The protein contains more than one transmebrane spanning domain so I can't answer this question.
   amino acids 1 - 48
   amino acids 10 - 32
   amino acids 47 - 72
   amino acids 73 - 300
5) When writing out a protein sequence we put which terminal on the right?
One answer only.
   The C-terminal
   The N-terminal
   It doesn't matter
   Both
   None of the above
Previous Page | Next Page