Coomassie blue: Difference between revisions
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Revision as of 20:02, 20 October 2014
Coomassie Brilliant Blue G-250 is a triphenylmethane which is commonly used as a dye to visuallise protein bands during PCR.
Methology of staining the polyacrylamide with the dye is as follows
- If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water.
- Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minuetes however will be at there peak of intensity within 1 hour.
- Repeat stage 1 to intesify the protein bands.
http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf