Coomassie blue

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Coomassie Brilliant Blue G-250[1] is a triphenylmethane which is commonly used as a dye to visuallise protein bands. 

Methology of staining the polyacrylamide with the dye is as follows:

  1. If the gel contains SDS then wash it 3 times for 5 minuetes each in deionised water.
  2. Cover the gel in Coosassie Brilliant Blue G-250 dye for 1 hour, the proteins bands will begin to appear within 5 minutes however will be at there peak of intensity within 1 hour.
  3. Repeat stage 1 to intesify the protein bands.

References

  1. http://www.gbiosciences.com/PDF/Protocol/Coomassie_Brilliant_Blue_G-250.pdf



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