Immunogold labelling: Difference between revisions
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Created page with "<u>'''Immunogold labelling'''</u> '''Immunogold labelling is a form of electron microscopy. This method is used in molecular biology to determine the distribution of protei..." |
Cleaned up the entry. Added in some links. Sorted out the references. Poorly formated. Didn't follow publication guidelines. |
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Immunogold labelling is a form of [[electron microscopy|electron microscopy]]. This method is used in molecular biology to determine the distribution of [[protein|proteins]] in a [[cell|cell]]. | |||
A thin sample is initially incubated with a [[primary antibody|primary antibody]]. A [[secondary antibody|secondary antibody]] is then added which is labelled with a gold nanoparticle. Gold nanoparticle labels are useful as they are very easy to spot as it is electron dense and is seen as a black dot on an electron microscope | |||
Using an array of antibodies in a [[yeast|yeast]] cell for example the protein in the spindle pole body can be localised. | |||
However, this method can give a misleading 2D image so further methods such as; tomography, z-stacks and single particle analysis can be used to create a more precise 3D image <ref>Molecular Biology of the Cell, Alberts et.al, 2008</ref>. | |||
=== References === | |||
<references /> | |||
<u>'''<br>'''</u> | <u>'''<br>'''</u> | ||
Latest revision as of 02:06, 24 October 2014
Immunogold labelling is a form of electron microscopy. This method is used in molecular biology to determine the distribution of proteins in a cell.
A thin sample is initially incubated with a primary antibody. A secondary antibody is then added which is labelled with a gold nanoparticle. Gold nanoparticle labels are useful as they are very easy to spot as it is electron dense and is seen as a black dot on an electron microscope
Using an array of antibodies in a yeast cell for example the protein in the spindle pole body can be localised.
However, this method can give a misleading 2D image so further methods such as; tomography, z-stacks and single particle analysis can be used to create a more precise 3D image [1].
References
- ↑ Molecular Biology of the Cell, Alberts et.al, 2008