Primary antibody

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Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies [1].

References

  1. Alberts et al. 2002 Molecular Biology of the cell 5(9)588-589
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