Electroporation: Difference between revisions
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Electroporation is a method used to insert a recombinant [[Plasmid|plasmid]] into a bacterial cell using high voltage electrical pulses<ref>http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html</ref>. | Electroporation is a method used to insert a recombinant [[Plasmid|plasmid]] into a bacterial cell using high voltage electrical pulses<ref>http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html</ref>. | ||
In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells<sup>[ | In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells<sup>[2]</sup> | ||
<sup></sup> | <sup></sup> | ||
<sup></sup><u>Example of electroporation:</u> | <sup></sup><u>Example of electroporation:</u> | ||
<u></u>E.coli plasmid insertion with CaCl2 on ice with heat shock. | <u></u>E.coli plasmid insertion with CaCl2 on ice with heat shock. | ||
[[Image: | [[Image:E coli-ed01.jpg]] | ||
<sub></sub>Figure 1: E.coli ( a common prokaryotic model organism into which recombinant plasmds are inserted) | <sub></sub>Figure 1: E.coli ( a common prokaryotic model organism into which recombinant plasmds are inserted) | ||
=== References === | === References === | ||
Revision as of 14:51, 16 November 2017
Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses[1].
In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells[2]
Example of electroporation:
E.coli plasmid insertion with CaCl2 on ice with heat shock.
Figure 1: E.coli ( a common prokaryotic model organism into which recombinant plasmds are inserted)
References
2.Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/