Electroporation

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E coli-ed01.jpg
Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses[1].

In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells[2].

Example of electroporation:

E. coli plasmid insertion with CaCl2 on ice with heat shock.

References

  1. http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html
  2. Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/

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