Morpholino: Difference between revisions
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Explanation of morpholinos: description and function |
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=== Description === | |||
*Small oligomer of synthetic [[Nucleotide|nucleotide]] analogues that block [[Gene expression|gene expression]]. | |||
*25 bp length | |||
=== Function === | |||
*attaches to [[MRNA|mRNA]], pre/mature, to cause [[Gene knockdown|gene knockdown]] | |||
*Used as gene knockdown tool on [[Zebra fish|Zebra fish]]<ref>Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.</ref> | |||
On | ==== On mature mRNA: ==== | ||
*attaches to AUG blocking [[Ribosome|ribosome]] interaction | |||
==== On pre-mRNA: ==== | |||
*sits on splice site, an [[Intron-exon junction|intron-exon junction]], making a double-stranded RNA, blocking [[Spliceosome|spliceosome]] which will create truncated/no [[Protein|protein]]<br> | |||
=== References === | |||
<references /> | |||
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Latest revision as of 17:17, 16 November 2017
Description
- Small oligomer of synthetic nucleotide analogues that block gene expression.
- 25 bp length
Function
- attaches to mRNA, pre/mature, to cause gene knockdown
- Used as gene knockdown tool on Zebra fish[1]
On mature mRNA:
- attaches to AUG blocking ribosome interaction
On pre-mRNA:
- sits on splice site, an intron-exon junction, making a double-stranded RNA, blocking spliceosome which will create truncated/no protein
References
- ↑ Shukla, Arun K., editor. “The Zebrafish: Disease Models and Chemical Screens.” Methods in Cell Biology, vol. 105, no. 12, 2011, pp. 273–308., doi:10.1016/c2009-0-62387-4.