Gene knockdown is a type of RNA interference which reduces the expression of a particular gene by degrading the mRNA for the protein it codes for. This can be achieved using small interfering RNAs (siRNAs), which are strands of dsRNA 21 to 23 nucleotides long and complementary to the mRNA in question. The siRNA recognise the homologous mRNA sequence and cleave the mRNA at roughly the midpoint in the sequence.
- ↑ Mocellin S, Provenzano M. RNA interference: learning gene knock-down from cell physiology. Journal of Translational Medicine. 2004;2:39. doi:10.1186/1479-5876-2-39.