DNA polymerase I: Difference between revisions
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DNA polymerase I is an enzyme that is involved in DNA replication in [[ | DNA polymerase I is an enzyme that is involved in [[DNA|DNA]] replication in ''[[Escherichia coli|Escherichia coli]]''. It is part of a group of [[DNA polymerase|DNA polymerases]] that copy [[DNA|DNA]]; they are highly coordinated in order to replicate whole genomes precisely and rapidly. DNA polymerase I is a slow, non-processive [[Enzyme|enzyme]] that fills the gaps between [[Okazaki fragment|Okazaki]] fragments of the lagging strand by Watson-Crick base pairing. The [[Enzyme|enzyme]] has two additional activites: | ||
#5’→3’ [[Exonuclease|exonuclease]] activity to remove RNA primers ahead of the polymerase site <ref>Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801</ref>. | |||
#3’→5’ [[Exonuclease|exonuclease]] activity for proof-reading and accuracy. | |||
=== References === | === References === | ||
<references /> | <references /> | ||
Latest revision as of 22:45, 27 November 2010
DNA polymerase I is an enzyme that is involved in DNA replication in Escherichia coli. It is part of a group of DNA polymerases that copy DNA; they are highly coordinated in order to replicate whole genomes precisely and rapidly. DNA polymerase I is a slow, non-processive enzyme that fills the gaps between Okazaki fragments of the lagging strand by Watson-Crick base pairing. The enzyme has two additional activites:
- 5’→3’ exonuclease activity to remove RNA primers ahead of the polymerase site [1].
- 3’→5’ exonuclease activity for proof-reading and accuracy.
References
- ↑ Berg J, Tymoczko J, Stryer L (2007) Biochemistry, 6th edition, New York: W.H. Freeman, p. 801