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HindIII is a [[Restriction Endonuclease|restriction endonuclease]] which is often used in [[Recombinant DNA Technology|recombinant technology]]. When the specific [[Palindromic sequence|palindromic]] base sequence of AAGCTT is present alongside [[Cofactor|cofactor]] Mg<sup>2+</sup> then HindIII will cleave the sequence<ref>D. Tang, S. Ando, Y. Takasaki, J. Tadano. "Mutational analyses of restriction endonuclease—HindIII mutant E86K with higher activity and altered specificity". Protein Engineering, Design and Selection, 2000. Volume 13, Issue 4, Pages 283–289.</ref><span style="font-size: 11.0667px;">.</span> <sup></sup> | HindIII is a [[Restriction Endonuclease|restriction endonuclease]] which is often used in [[Recombinant DNA Technology|recombinant technology]]. When the specific [[Palindromic sequence|palindromic]] base sequence of AAGCTT is present alongside [[Cofactor|cofactor]] Mg<sup>2+</sup> then HindIII will cleave the sequence<ref>D. Tang, S. Ando, Y. Takasaki, J. Tadano. "Mutational analyses of restriction endonuclease—HindIII mutant E86K with higher activity and altered specificity". Protein Engineering, Design and Selection, 2000. Volume 13, Issue 4, Pages 283–289.</ref><span style="font-size: 11.0667px;">.</span> <sup></sup><br> | ||
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Latest revision as of 16:23, 4 December 2017
HindIII is a restriction endonuclease which is often used in recombinant technology. When the specific palindromic base sequence of AAGCTT is present alongside cofactor Mg2+ then HindIII will cleave the sequence[1].
References:
- ↑ D. Tang, S. Ando, Y. Takasaki, J. Tadano. "Mutational analyses of restriction endonuclease—HindIII mutant E86K with higher activity and altered specificity". Protein Engineering, Design and Selection, 2000. Volume 13, Issue 4, Pages 283–289.