SDS-PAGE: Difference between revisions
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Created page with "This separates proteins by virtue of mass. This is done under denaturing conditions as SDS denatures protein. The SDS also adds negative chage to the proteins which ..." |
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See [[SDS polyacrylamide-gel electrophoresis|SDS polyacrylamide-gel electrophoresis]] | |||
< | SDS (Sodium Dodecyl Sulphate), a negatively charged substance or anionic [[Surfactant|surfactant]] with a sulfate group attached, allows [[Proteins|proteins]] of all charges to travel down the [[Polyacrylamide|polyacrylamide]] to the positive electrode<ref>Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/</ref>. This SDS will bind to [[Protien|proteins]] and disrupt non-covalent bonds, which causes [[Denature|denaturation]] in proteins. Once the protein is bound to the SDS molecule it will become negatively charged. | ||
=== References === | |||
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Latest revision as of 06:36, 22 October 2018
See SDS polyacrylamide-gel electrophoresis
SDS (Sodium Dodecyl Sulphate), a negatively charged substance or anionic surfactant with a sulfate group attached, allows proteins of all charges to travel down the polyacrylamide to the positive electrode[1]. This SDS will bind to proteins and disrupt non-covalent bonds, which causes denaturation in proteins. Once the protein is bound to the SDS molecule it will become negatively charged.
References
- ↑ Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/