SDS-PAGE

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See SDS polyacrylamide-gel electrophoresis

SDS (Sodium Dodecyl Sulphate), a negatively charged substance or anionic surfactant with a sulfate group attached, allows proteins of all charges to travel down the polyacrylamide to the positive electrode[1]. This SDS will bind to proteins and disrupt non-covalent bonds, which causes denaturation in proteins. Once the protein is bound to the SDS molecule it will become negatively charged.

References

  1. Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/
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