SDS-PAGE: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (4 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
See | See [[SDS polyacrylamide-gel electrophoresis|SDS polyacrylamide-gel electrophoresis]] | ||
SDS (Sodium Dodecyl Sulphate), a negatively charged substance or anionic [[Surfactant|surfactant]] with a sulfate group attached, allows [[Proteins|proteins]] of all charges to travel down the [[Polyacrylamide|polyacrylamide]] to the positive electrode<ref>Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/</ref>. This SDS will bind to [[Protien|proteins]] and disrupt non-covalent bonds, which causes [[Denature|denaturation]] in proteins. Once the protein is bound to the SDS molecule it will become negatively charged. | |||
=== References === | |||
<references /> | |||
Latest revision as of 06:36, 22 October 2018
See SDS polyacrylamide-gel electrophoresis
SDS (Sodium Dodecyl Sulphate), a negatively charged substance or anionic surfactant with a sulfate group attached, allows proteins of all charges to travel down the polyacrylamide to the positive electrode[1]. This SDS will bind to proteins and disrupt non-covalent bonds, which causes denaturation in proteins. Once the protein is bound to the SDS molecule it will become negatively charged.
References
- ↑ Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Isolating, Cloning, and Sequencing DNA. Available from: https://www.ncbi.nlm.nih.gov/books/NBK26837/