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Pfu polymerase is an enzyme isolated from the organism ''[[Pyrococcus furiosus|Pyrococcus furiosus]]'', a [[Hyperthermophilic archaeon|hyperthermophilic archaeon]]. This [[Organism|organism]] can survive temperatures of up to one hundred degrees celcius, therefore is very apt for use in the [[PCR|PCR]] reaction, as, unlike [[DNA Polymerase|DNA polymerase]], it can survive and function at the high temperatures the process operates at. It may be preferred to the use of [[Taq polymerase|Taq polymerase ]](another thermostable enzyme) as pfu has 3'-5' proof reading activity, improving the accuracy of copying [[DNA|DNA]] | Pfu polymerase is an enzyme isolated from the organism ''[[Pyrococcus furiosus|Pyrococcus furiosus]]'', a [[Hyperthermophilic archaeon|hyperthermophilic archaeon]]. This [[Organism|organism]] can survive temperatures of up to one hundred degrees celcius, therefore is very apt for use in the [[PCR|PCR]] reaction, as, unlike [[DNA Polymerase|DNA polymerase]], it can survive and function at the high temperatures the process operates at. It may be preferred to the use of [[Taq polymerase|Taq polymerase]] (another thermostable enzyme) as pfu has 3'-5' proof reading activity, improving the accuracy of copying [[DNA|DNA]]<ref name="null">Gerhard Fiala, Karl O. Stetter "Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C" Archives of Microbiology 1986 145: 56–61</ref>.<br> | ||
=== References === | |||
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Latest revision as of 16:18, 6 December 2018
Pfu polymerase is an enzyme isolated from the organism Pyrococcus furiosus, a hyperthermophilic archaeon. This organism can survive temperatures of up to one hundred degrees celcius, therefore is very apt for use in the PCR reaction, as, unlike DNA polymerase, it can survive and function at the high temperatures the process operates at. It may be preferred to the use of Taq polymerase (another thermostable enzyme) as pfu has 3'-5' proof reading activity, improving the accuracy of copying DNA[1].
References
- ↑ Gerhard Fiala, Karl O. Stetter "Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C" Archives of Microbiology 1986 145: 56–61