Vector: Difference between revisions
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A Vector acts as a template for the [[Cloning|cloning]] of a specific [[Genes|gene]]. This gene needs to be inserted into the vector by [[Recombinant DNA Technology|recombinant DNA]], before the vector can be inserted into a target [[Host cell|host cell]] | A Vector acts as a template for the [[Cloning|cloning]] of a specific [[Genes|gene]]. This gene needs to be inserted into the vector by [[Recombinant DNA Technology|recombinant DNA]], before the vector can be inserted into a target [[Host cell|host cell]]<ref>Hartl D and Ruvolo M (2012). Genetics, Analysis of Genes and Genomes. 8th Edition. The United States. Jones and Bartlett Learning.</ref>. | ||
Vectors are essential components for recombinant [[DNA|DNA]] technology. They act as the vehicle to carry [[Recombinant DNA Technology|recombinant DNA]] into the target host [[Cell|cell]]. | Vectors are essential components for recombinant [[DNA|DNA]] technology. They act as the vehicle to carry [[Recombinant DNA Technology|recombinant DNA]] into the target host [[Cell|cell]]. | ||
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Vectors have special [[Restriction site|restriction]] sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient [[Origin of replication|origin of replication]]. | Vectors have special [[Restriction site|restriction]] sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient [[Origin of replication|origin of replication]]. | ||
The most common types of vectors are | The most common types of vectors are [[Plasmid|plasmid]] (which are circular pieces of DNA naturally found in [[Bacteria|bacteria]])<ref>Genetics: analysis of genes and genomes (2009), Hartl and Jones, 7th Ed. Jones and Bartlett</ref>, viruses and [[Cosmid|cosmids]] (a [[Plasmid|plasmid]] containing [[Cos sites|cos sites]]). A plasmid can be linear or circular and is always double-stranded. | ||
=== References === | === References === | ||
<references / | <references /> | ||
Latest revision as of 10:04, 8 December 2018
A Vector acts as a template for the cloning of a specific gene. This gene needs to be inserted into the vector by recombinant DNA, before the vector can be inserted into a target host cell[1].
Vectors are essential components for recombinant DNA technology. They act as the vehicle to carry recombinant DNA into the target host cell.
Vectors have special restriction sites onto which the recombinant DNA can insert itself. They are relatively easy to manipulate and introduce into the target host cell. Another important property of vectors is that they can be an efficient origin of replication.
The most common types of vectors are plasmid (which are circular pieces of DNA naturally found in bacteria)[2], viruses and cosmids (a plasmid containing cos sites). A plasmid can be linear or circular and is always double-stranded.