Immunolabelling: Difference between revisions
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Immunolebelling is a technique used to detect [[Proteins|proteins]] this could in a cell or in an expertiment such as [[ | Immunolebelling is a technique used to detect [[Proteins|proteins]] this could in a cell or in an expertiment such as [[Western Blotting|Western Blotting]]. An [[Antibody|antibody]] that will recongise that your desired protein is added, in case of a [[Cell|cell]] a method to get the antibody into the cell must be deveopled. The antibody ([[Primary antibody|primary antibody]]) will specifically bind to the [[Antigen|antigen]]. In a Western Blottting experiment this take about an one hour and half on a moving plate. Then you add a [[Secondary antibody|secondary antibody]] which recongises the primary antibody as a antigen. The secondary antibody is conjugated with [[Fluorescent marker|fluorescent marker]]. So the location of your protein can be detected using this technique <ref>Giepmans et al (2006) et al 312 , 217-223</ref>. | ||
=== References === | === References === | ||
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Revision as of 10:41, 16 November 2011
Immunolebelling is a technique used to detect proteins this could in a cell or in an expertiment such as Western Blotting. An antibody that will recongise that your desired protein is added, in case of a cell a method to get the antibody into the cell must be deveopled. The antibody (primary antibody) will specifically bind to the antigen. In a Western Blottting experiment this take about an one hour and half on a moving plate. Then you add a secondary antibody which recongises the primary antibody as a antigen. The secondary antibody is conjugated with fluorescent marker. So the location of your protein can be detected using this technique [1].
References
- ↑ Giepmans et al (2006) et al 312 , 217-223