Electrophoretic mobility shift assay: Difference between revisions
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Electrophoretic mobility shift assay (also known as the EMSA) is an affinity-electrophoresis technique used to detect the binding of DNA-protein complexes.The Electrophoretic mobility is normally reduced when the DNA cognates to protein,causing a consequent shift in the region of the fragment band.This method is usually used to detect the transcription factor in protein, using DNA fragments containing regulatory element. | Electrophoretic mobility shift [[assay|assay]] (also known as the EMSA) is an [[affinity-electrophoresis|affinity-electrophoresis]] technique used to detect the binding of [[DNA-protein complexes|DNA-protein complexes]].The Electrophoretic mobility is normally reduced when the [[DNA|DNA]] cognates to [[protein|protein]], causing a consequent shift in the region of the fragment band. This method is usually used to detect the [[transcription factor|transcription factor]] in protein, using DNA fragments containing regulatory element <ref>p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh</ref>. | ||
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Revision as of 21:32, 24 November 2011
Electrophoretic mobility shift assay (also known as the EMSA) is an affinity-electrophoresis technique used to detect the binding of DNA-protein complexes.The Electrophoretic mobility is normally reduced when the DNA cognates to protein, causing a consequent shift in the region of the fragment band. This method is usually used to detect the transcription factor in protein, using DNA fragments containing regulatory element [1].
References
- ↑ p.286-287 Molecular Cell Biology book.Authors. H.Lodish,A.Berk,C.A.Kaiser,M.Krieger,M.P.Scott,A.Bretscher,H.Ploegh