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DNA helicase is an enzyme responsible for the separation of two annealed strands of nucleic acids. This process allows a single-stranded [[DNA|DNA]] to form and be used as a template during [[Transcription|transcription]]. ATP hydrolysis provides the energy for the enzyme to translocate along the double helix and generate a replication fork<ref>Alberts, Johnson, Lewis, Raff, Roberts, Walters. (2008) Molecular Biology of the Cell: 273, 5th edition, New York: Garland Science.</ref>. In [[ | DNA helicase is an [[enzyme|enzyme]] responsible for the separation of two annealed strands of [[nucleic acids|nucleic acids]]. This process allows a single-stranded [[DNA|DNA]] to form and be used as a template during [[Transcription|transcription]]. ATP hydrolysis provides the energy for the enzyme to translocate along the double helix and generate a replication fork<ref>Alberts, Johnson, Lewis, Raff, Roberts, Walters. (2008) Molecular Biology of the Cell: 273, 5th edition, New York: Garland Science.</ref>. In [[Escherichia_coli|''E. coli'']] DNA helicase is called the [[DnaB protein|DnaB protein]], and it differs slightly in the way it carries out it's function. DnaB forms a three-fold ring structure, with the lagging strand binding in the central region, whilst the leading strand is excluded. The DnaB translocates through a mechanism where dNTP's bind and cause a conformational change, forcing the DNA strands to separate<ref>Alberts, Johnson, Lewis, Raff, Roberts, Walters. (2008) Molecular Biology of the Cell: 283, 5th edition, New York: Garland Science.</ref>. <br> | ||
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Revision as of 18:59, 28 November 2011
DNA helicase is an enzyme responsible for the separation of two annealed strands of nucleic acids. This process allows a single-stranded DNA to form and be used as a template during transcription. ATP hydrolysis provides the energy for the enzyme to translocate along the double helix and generate a replication fork[1]. In E. coli DNA helicase is called the DnaB protein, and it differs slightly in the way it carries out it's function. DnaB forms a three-fold ring structure, with the lagging strand binding in the central region, whilst the leading strand is excluded. The DnaB translocates through a mechanism where dNTP's bind and cause a conformational change, forcing the DNA strands to separate[2].