Blunt ends: Difference between revisions
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Symmetrical cleavage occurs when a [[Restriction enzyme|restriction endonuclease]], such as HaeIII, cuts a section of [[DNA|DNA]] and leaves no overhanging bases (known as [[‘sticky’ ends|Sticky Ends]]). The enzyme cuts between two bases directly opposite each other on the complementary strands of the double stranded [[DNA|DNA]]. This cleavage results in the formation | Symmetrical cleavage occurs when a [[Restriction enzyme|restriction endonuclease]], such as HaeIII, cuts a section of [[DNA|DNA]] and leaves no overhanging bases (known as [[‘sticky’ ends|Sticky Ends]]). The enzyme cuts between two bases directly opposite each other on the complementary strands of the double stranded [[DNA|DNA]]. This cleavage results in the formation of blunt ended DNA fragments. | ||
Revision as of 14:41, 21 October 2012
Symmetrical cleavage occurs when a restriction endonuclease, such as HaeIII, cuts a section of DNA and leaves no overhanging bases (known as Sticky Ends). The enzyme cuts between two bases directly opposite each other on the complementary strands of the double stranded DNA. This cleavage results in the formation of blunt ended DNA fragments.