Primary antibody: Difference between revisions
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Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies. Alberts et al, Molecular Biology of the cell, 5, (9)588-589 | |||
Revision as of 19:06, 21 October 2013
Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies. Alberts et al, Molecular Biology of the cell, 5, (9)588-589