Immunolabelling: Difference between revisions
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Immunolabelling is a technique used to detect [[Proteins|proteins]] either in a cell or in an experiment such as [[Western Blotting|Western Blotting]]. An [[Antibody|antibody]] will recognise when the desired protein is added, in the case of a [[Cell|cell]] a method must be devised to get the antibody inside. The antibody ([[Primary antibody|primary antibody]]) specifically binds to the [[Antigen|antigen]]. In a Western Blotting experiment this takes about an hour and a half on a moving plate. Then you add a [[Secondary antibody|secondary antibody]] which recognises the primary antibody as an antigen. The secondary antibody is conjugated with a [[Fluorescent marker|fluorescent marker]], so the location of your protein can be detected using this technique. | |||
Latest revision as of 07:03, 23 October 2018
Immunolabelling is a technique used to detect proteins either in a cell or in an experiment such as Western Blotting. An antibody will recognise when the desired protein is added, in the case of a cell a method must be devised to get the antibody inside. The antibody (primary antibody) specifically binds to the antigen. In a Western Blotting experiment this takes about an hour and a half on a moving plate. Then you add a secondary antibody which recognises the primary antibody as an antigen. The secondary antibody is conjugated with a fluorescent marker, so the location of your protein can be detected using this technique.