Primary antibody: Difference between revisions
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Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies | Primary antibodies can be used in conjunction with [[Secondary_antibody|secondary antibodies]] as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies <ref>Alberts et al. 2002 Molecular Biology of the cell 5(9)588-589</ref>. | ||
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Latest revision as of 01:13, 22 October 2013
Primary antibodies can be used in conjunction with secondary antibodies as an extrinsic label in microscopic imaging. The primary antibodies are infused into a permeabilised cell/ tissue. They react with a complementary secondary antibodies which are fluorescently labelled. This technique is called immunoflourescence microscopy which is used in protein localisation studies [1].
References
- ↑ Alberts et al. 2002 Molecular Biology of the cell 5(9)588-589