Restriction enzymes: Difference between revisions

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 Restriction Enzymes, or Restriction Endonucleases cut DNA into smaller fragments (Alberts et al., 2008).
Restriction Enzymes, or Restriction Endonucleases cut DNA into smaller fragments <ref>Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science</ref>.


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Different restriction enzymes recognise different squences, normally between 4 to 8 nucleotides in length. Some restriction enzymes produce staggered or [[Sticky_ends|'sticky' ends]] while others leave [[Blunt_ends|blunt ends]]<ref>Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science</ref>. Examples of restriction enzymes that produce staggered ends are [[EcoR1|EcoR1]] and [[Hind111|Hind111]]<ref>Dryden, D.T.F., Loenen, W.A.M., Murray, N.E., Raleigh, E.A., Wilson, G.G., 2013. Highlights of the DNA Cutters: a short history of restriction enzymes. Nucleic Acids Research, 42(1), pp 3-19.</ref>. Hpal is an example of a restriction enzyme that forms blunt ends <ref>Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science</ref>.&nbsp;


Different restriction enzymes recognise different squences, normally between 4 to 8 nucleotides in length. Some restriction enzymes produce staggered or 'sticky' ends while others leave blunt ends&nbsp;(Alberts et al., 2008). Examples of restriction enzymes that produce staggered ends are EcoR1 and Hind111 (Dryden et al., 2013). Hpal is an example of a restriction enzyme that forms blunt ends (Alberts et al., 2008).&nbsp;
Restriction Enzymes are often used to create recombinant DNA molecules. See&nbsp;[[Recombinant DNA Technology]].<br>


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=== References<br> ===


Restriction Enzymes are often used to create recombinant DNA molecules. See&nbsp;[[Recombinant_DNA_Technology]].
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'''References'''
 
Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science
 
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Dryden, D.T.F., Loenen, W.A.M., Murray, N.E., Raleigh, E.A., Wilson, G.G., 2013. Highlights of the DNA Cutters: a short history of restriction enzymes. ''Nucleic Acids Research'', 42(1), pp 3-19.
 
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Revision as of 09:00, 22 October 2014

Restriction Enzymes, or Restriction Endonucleases cut DNA into smaller fragments [1].

Different restriction enzymes recognise different squences, normally between 4 to 8 nucleotides in length. Some restriction enzymes produce staggered or 'sticky' ends while others leave blunt ends[2]. Examples of restriction enzymes that produce staggered ends are EcoR1 and Hind111[3]. Hpal is an example of a restriction enzyme that forms blunt ends [4]. 

Restriction Enzymes are often used to create recombinant DNA molecules. See Recombinant DNA Technology.

References

  1. ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science
  2. ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science
  3. ↑ Dryden, D.T.F., Loenen, W.A.M., Murray, N.E., Raleigh, E.A., Wilson, G.G., 2013. Highlights of the DNA Cutters: a short history of restriction enzymes. Nucleic Acids Research, 42(1), pp 3-19.
  4. ↑ Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., 2008. Molecular Biology of the Cell. 5th Ed. New York: Garland Science


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