DNA Sequencing: Difference between revisions
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The advent of techniques for the rapid sequencing of [[DNA|DNA]] has led to many major advances in molecular biology. Several methods have now been developed for determining the nucleotide sequence of [[DNA|DNA]], but the [[Sanger “dideoxy” method|Sanger (“dideoxy”) method]] offers significant advantages in terms of rapidity and simplicity of protocol. It is based upon the use of [[ | The advent of techniques for the rapid sequencing of [[DNA|DNA]] has led to many major advances in molecular biology. Several methods have now been developed for determining the nucleotide sequence of [[DNA|DNA]], but the [[Sanger “dideoxy” method|Sanger (“dideoxy”) method]] offers significant advantages in terms of rapidity and simplicity of protocol. It is based upon the use of [[Deoxynucleotide analogues|deoxynucleotide analogues]] that are randomly incorporated into a growing [[DNA|DNA]] strand to give specific chain termination<ref>James M. Heather⁎ and Benjamin Chain. The sequence of sequencers: The history of sequencing DNA. Genomics. 2016 Jan; 107(1): 1–8.</ref>. | ||
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Latest revision as of 12:31, 5 December 2018
The advent of techniques for the rapid sequencing of DNA has led to many major advances in molecular biology. Several methods have now been developed for determining the nucleotide sequence of DNA, but the Sanger (“dideoxy”) method offers significant advantages in terms of rapidity and simplicity of protocol. It is based upon the use of deoxynucleotide analogues that are randomly incorporated into a growing DNA strand to give specific chain termination[1].
References
- ↑ James M. Heather⁎ and Benjamin Chain. The sequence of sequencers: The history of sequencing DNA. Genomics. 2016 Jan; 107(1): 1–8.