Standard curve: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
A Standard curve is a way of plotting data on a graph. It is used to determine relationship between different variables in a data set. | A Standard curve is a way of plotting data on a graph. It is used to determine relationship between different variables in a data set. The standard curve is used to determine the value of the unknown variable from the easily measurible variable.<br> | ||
If the procedure follows [[Beer-Lambert Law|Beer's law]], then the plotted points would lie in such a way that a straight line can be drawn through them , this will help to determine the unknown values by locating their %T/Abs on the line then drawing a line to intersect the point on the line and then extending it to the concentration axis<sup>[1]</sup>. | |||
the formula for Beer's law is: | |||
A= e x l x c | |||
A= Absorbance | |||
E= molar absorbtivity constant | |||
L= path length | |||
C= concentration | |||
<br> | |||
References<br> Thermo fisher scientific. How to use protein assay standard curve. 2012. [cited: 05/12/17] | |||
Available from: https://tools.thermofisher.com/content/sfs/brochures/TR0057-Read-std-curves.pdf | |||
Revision as of 23:49, 5 December 2017
A Standard curve is a way of plotting data on a graph. It is used to determine relationship between different variables in a data set. The standard curve is used to determine the value of the unknown variable from the easily measurible variable.
If the procedure follows Beer's law, then the plotted points would lie in such a way that a straight line can be drawn through them , this will help to determine the unknown values by locating their %T/Abs on the line then drawing a line to intersect the point on the line and then extending it to the concentration axis[1].
the formula for Beer's law is:
A= e x l x c
A= Absorbance
E= molar absorbtivity constant
L= path length
C= concentration
References
Thermo fisher scientific. How to use protein assay standard curve. 2012. [cited: 05/12/17]
Available from: https://tools.thermofisher.com/content/sfs/brochures/TR0057-Read-std-curves.pdf